Journal: PLoS Pathogens

Article Title: Homeostatic Proliferation Fails to Efficiently Reactivate HIV-1 Latently Infected Central Memory CD4+ T Cells

doi: 10.1371/journal.ppat.1002288

Figure Lengend Snippet: (A) Mock or DHIV latently infected, cultured T CM were left in the absence of cytokines (None) or in the presence of IL-2, IL-7 or a combination of IL-2 and IL-7 (IL-2+IL-7) or costimulated with antibodies to CD3 and CD28 (αCD3/αCD28) and assessed for intracellular p24Gag and Ki67 expression by flow cytometry. Numbers in boxes indicate percentages. (B) Analysis corresponding to 4 different donors analyzed as shown in panel A; each donor is represented with a different symbol and horizontal lines indicate media values. Statistical significance was calculated by 2-tailed paired-sample t test analysis (P values provided, N.S. not significant). (C) Analysis of the percentage of p24Gag + cells in each compartment (Ki67 − or Ki67 + ) after IL-2+IL-7 treatment in the four donors analyzed in B. Data was normalized as indicated in the text. Horizontal lines indicate media values. Statistical significance was calculated by 2-tailed paired-sample t test analysis (N.S. not significant).

Article Snippet: Cells were then washed with Perm/Wash Buffer and incubated with a 1/20 dilution of anti-Ki67-PE (BD Biosciences) in 100 µl of Perm/Wash Buffer for 30 min at 4°C.

Techniques: Infection, Cell Culture, Expressing, Flow Cytometry